Appendix.
Antibody Table
| Peptide/Protein Target | Name of Antibody | Manufacturer, Catalog No. | Species Raised in | Dilution Used | RRID |
|---|---|---|---|---|---|
| KLOTHO | R&D Systems, AF1819 | Goat | 15 μg/mL for immunohistochemistry, 0.1 μg/mL for Western blot | AB_2296612 | |
| pERK | Cell Signaling Technology, Inc., 9106 | Mouse | at 1:150 for immunohistochemistry, 1:1000 for Western blot | AB_331768 | |
| NPT2a | Alpha Diagnostic International, Inc., NPT23-A | Rabbit | 20 μg/mL for immunohistochemistry, 1 μg/mL for Western blot | AB_1622108 | |
| ERK | Cell Signaling Technology, Inc., 9102 | Rabbit | at 1:1000 for Western blot | AB_330744 | |
| Actin | Santa Cruz Biotechnology, sc-1615 | Goat | at 1:10,000 for Western blot | AB_630835 |