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Single-label ISH for ERα mRNA expression in AVPV/PeN in male and female mice. (A) Representative photomicrographs of ERα in the AVPV/PeN in E2-treated male and female mice at CT12. Female mice have a considerably higher degree of ERα in the AVPV/PeN than do males. (B) Quantification of the gene expression of ERα in the AVPV/PeN of E2-treated males and females. Different lowercase letters indicate significantly different groups (P < 0.05).
