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(a) Immunofluorescent staining of MCF10A cells for YAP (green) and DAPI (blue) illustrating the subcellular localization of YAP for the monolayer migrating on secondary ECM, after priming. Scale bar = 50 μm. (b,c) Average nuclear-to-cytoplasmic ratio of the YAP fluorescent intensity for MCF10A, MCF7, and A431 cells within the monolayer. *p3<30.05 with respect to control ECMs of homogeneous stiffness. Error bars = SEM. N>40.
