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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Inflamm Bowel Dis. 2017 Nov;23(11):1972–1982. doi: 10.1097/MIB.0000000000001273

Figure 3. Epicutaneous tolerance abrogates colitis in the DSS model.

Figure 3

A. Experimental protocol: C57BL/6 mice were exposed once to OVA on the skin and then given 5 days of 2% DSS in the drinking water followed by 5 days of normal water. B. Weight curves for mice exposed to OVA (+ET) or not (DSS) prior to DSS treatment. C. Histological scores for colons from mice exposed to OVA (+ET) or not (DSS) prior to DSS treatment and median histological scoring of colon specimens with ranges in parenthesis from mice exposed to OVA (+ET) or not (DSS) prior to DSS treatment shown under a representative H&E sections of colon demonstrating less severe inflammation and ulceration in mice exposed to OVA (scale bar = 100µm). D. Cytokine production as measured by ELISA from supernatants of colon cultures from mice exposed to OVA (+ET) or not (DSS) prior to DSS treatment. E. Colonic expression of regulatory markers as measured by rtPCR from mice exposed to OVA (+ET) or not (DSS) prior to DSS treatment. (n=2 independent experiments of 4–5 mice/group) (*p≤0.05, **p≤0.01, ***p≤0.001)