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Preparation of Pax7-tdTomato+ cells from mice. (A) Experimental flowchart. Briefly, Pax7iCreERT2;ROSA26LSLtdTomato mice were injected with tamoxifen to label Pax7+ SCs. Single FACS isolated SCs were captured and subjected to RNA-sequencing. Comparative bioinformatics analyses were performed using single cell transcriptomes. (B) Gating strategy for isolation of Pax7-tdTomato+ SCs by flow cytometry.
