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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2017 Oct 11;41(11):1875–1885. doi: 10.1111/acer.13496

Figure 3. Alcohol exposure in vitro attenuated Nrf2 and augmented TGFβ1 gene expression in murine primary lung fibroblasts.

Figure 3

Murine primary lung fibroblasts (PLFs) were cultured ± alcohol (60 mM) for 24-72 hours and messenger RNA was isolated at 24, 48, and 72 hours. (A) Consistent with our previous findings, alcohol treatment suppressed Nrf2 gene expression starting at 48 hours, and gene expression remained suppressed at 72 hours (as reflected by Nrf2 mRNA levels, normalized to 18s) compared to untreated cells. (B) Similarly, alcohol treatment augmented TGFβ1 gene expression at 24 and 48 hours compared to untreated cells. However, there was a decrease in TGFβ1 gene expression at 72 hours. *P<0.05 compared to untreated groups.