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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2017 Oct 11;41(11):1875–1885. doi: 10.1111/acer.13496

Figure 6. Sulforaphane attenuated miR-21 gene expression in alcohol-treated murine primary lung fibroblasts.

Figure 6

Murine primary lung fibroblasts (PLFs) were treated in vitro ± alcohol (Alc; 60 mM) ± sulforaphane (SFP; 5μM). Large and small RNA and protein were isolated at 48 hours and 72 hours, respectively. (A) Alcohol treatment significantly suppressed some of the Nrf2-dependent enzymes including glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and glutathione S-transferase theta 2 (GSTT2) gene expression, but not heme oxygenase-1 (HO-1). Addition of SFP significantly increased gene expression of all Nrf2-dependent enzymes (as reflected by mRNA levels, normalized to 18s). (B) At 48 hours, alcohol treatment increased miR-21 gene expression while an addition of SFP attenuated the effect of alcohol on miR-21 in PLFs (normalized to SNORD95). *P<0.05 compared to untreated groups.