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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Arch Biochem Biophys. 2017 Sep 18;633:93–102. doi: 10.1016/j.abb.2017.09.009

Figure 3.

Figure 3

Modulation of RetSat expression affects the formation of TBARS in response to tert-BHP. (A) Dose-response of the TBARS levels in response to tert-BHP in NIH/3T3 cells transfected with siRNA directed to RetSat (black circles) or with non-targeting control siRNAs (white circles). (B) Dose-response of the TBARS levels in response to tert-BHP in HEKK-RetSat cells induced with tetracycline (labeled RetSat Ovexp, black circles,) or not (labeled Ctr, white circles). (C) Dose-response of the TBARS levels in response to tert-BHP in MEFs from RetSat WT (white circles) or KO (black circles). (D) Levels of H2O2 released in the media by HEKK-RetSat cells either induced (black bar) or not (white bar) with tetracycline and represented as nmol/ gram protein. Comparison of the TBARS or H2O2 levels measured in control cells versus RetSat-suppressed or RetSat-deficient, or RetSat -overexpressing cells for a given dose of tert-BHP: * p<0.05, ** p<0.01, ***p<0.001. Data are normalized to protein levels and presented as mean ± sd (n=3).