Skip to main content
. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Transl Res. 2017 Jul 22;189:1–12. doi: 10.1016/j.trsl.2017.07.006

Figure 1. Mosaic of 80 image volumes collected from a mouse kidney thick section.

Figure 1

A 50 μm section of mouse kidney was cut with a vibratome and stained for nuclei (DAPI,blue), F-actin (green), and with an antibody against MHCII (red). Image volumes were collected using a 20X, NA 0.75 Leica objective on a Leica SP8 confocal microscope and mosaic merged in Fiji. Image field is 6 mm across. Inset – magnified region showing MHCII cells adjacent to two glomeruli.