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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Respir Physiol Neurobiol. 2017 Apr 27;256:97–108. doi: 10.1016/j.resp.2017.04.010

Figure 4. Thirty days of CIH following Blm injury increased average area of α-Smooth Muscle Actin positive staining relative to total lung parenchymal tissue area.

Figure 4

(A–D): Representative α-SMA immunostained images of Sal/Norm (A), Sal/CIH (B), Blm/Norm (C) and Blm/CIH (D) animals, 35 days after Sal or Blm treatment (20×). Emphysema-like changes are present in CIH treated animals (B, D) and increased foci of α-SMA+ staining (D; arrows) are seen in the lungs of Blm/CIH treated rats. Scale bar (shown in Panel A): 100 μm. (E): Average α-SMA+ region area (μm2). (F): Average SMA+ region area divided by total parenchymal tissue area in the microscope field, to account for presence of “emphysema-like” areas. Each symbol represents the average of all images (≥15) from one rat.