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Decreased mitochondrial enzymatic activity in VPS35 D620N fibroblasts. Specific enzymatic activities of complex I (CI) and II (CII) and citrate synthase (CS) were measured in isolated mitochondria from fibroblasts. (A) Enzymatic activity normalized to protein concentration. (B, C) Enzymatic activity normalized to citrate synthase activity. Data are presented as mean ± SEM from 3 independent experiments. Asterisks indicate statistical significance. ***p<0.001, ****p<0.0001.
