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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jul 29;1863(11):2791–2795. doi: 10.1016/j.bbadis.2017.07.032

Figure.3.

Figure.3

VPS35 D620N decreases levels of assembled Complex I and II which is rescued by mdivi-1 treatment. (A) Mitochondria isolated from fibroblasts were subjected to SDS-PAGE and probed with anti-OXPHOS antibody. VDAC1 was used as an internal loading control. (B) Mitochondria isolated from fibroblasts were subjected to BN-PAGE. CI, complex I; CII, complex II. (C) Quantitative analysis of band intensity normalized to the level of NHF+DMSO. Data corresponds to mean ± SEM from 3 independent experiments. Asterisks indicate statistical significance. *p<0.05, **p<0.01.