Table 2.

Antibody brain concentrations (nM) after i.c.v. infusion or four weekly i.v. bolus doses in cynomolgus monkeys

		anti‐BACE1 i.c.v.a				Control IgG i.c.v.a				anti‐BACE1 i.v.b
		Left hemisphere		Right hemisphere		Left hemisphere		Right hemisphere		Left hemisphere		Right hemisphere
Slicec	Brain regiond	Average	SEM	Average	SEM	Average	SEM	Average	SEM	Average	SEM	Average	SEM
Slice I	Prefrontal cortex	41.0	21.0	53.7	14.3	41.0	4.55	33.0	1.24	1.96	0.505	1.41	0.242
	Cingulate cortex	33.5	7.42	36.6	3.73	69.5	20.4	25.0	0.983	0.727	0.0492	0.969	0.168
Slice II	Prefrontal cortex	31.1	4.79	24.3	7.93	38.1	10.5	41.7	7.75	0.627	0.135	0.543	0.0927
	Caudate	65.8	17.9	28.4	13.6	123	53.1	37.6	21.6	0.562	0.0703	0.631	0.153
Slice III	Cingulate cortex	30.0	5.69	19.8	7.31	32.1	7.1.0	37.3	15.0	0.745	NA	0.984	0.157
	Motor cortex	37.4	10.6	53.9	10.3	27.7	5.22	35.9	2.66	0.722	0.080	0.899	0.171
	Somatosensory and insular cortex	39.5	4.19	44.6	7.42	46.3	7.27	42.2	6.8.0	0.885	NA	0.779	0.183
	Temporal cortex	38.2	9.45	30.3	10.0	38.7	4.69	43.1	3.08	0.945	0.160	0.885	0.155
	Entorhinal cortex	44.2	19.2	46.7	15.7	41.4	5.64	49.3	4.08	0.882	NA	0.816	0.165
	Caudate	22.2	12.0	16.7	9.23	27.0	4.83	7.17	3.17	0.674	0.100	0.642	0.0958
	Putamen	10.2	8.31	1.85	0.698	12.2	7.05	6.59	2.69	0.566	0.0723	0.452	0.0451
	Globus pallidus	16.4	14.7	5.86	4.08	16.5	10.6	4.71	2.11	0.392	0.0839	0.371	0.0718
	Hypothalamus	35.0	7.14	11.5	5.99	44.8	19.0	24.1	9.56	0.709	0.133	0.731	0.247
	Amygdala	23.2	5.78	15.5	4.2.0	34.0	7.57	32.7	8.87	1.28	0.648	1.02	0.482
Slice IV	Cingulate cortex	42.9	5.04	33.7	5.39	36.1	4.99	44.0	8.92	0.689	0.0182	0.936	0.185
	Parietal cortex	27.1	4.15	16.0	5.71	38.1	5.06	35.9	4.63	0.494	0.0850	0.841	0.117
	Temporal cortex	38.3	8.2.0	38.7	13.8	48.5	8.08	40.7	6.06	1.03	0.396	0.974	0.307
	Thalamus	27.7	9.35	34.1	16.9	28.0	5.10	32.9	12.0	1.37	0.788	0.879	0.200
	Hippocampus	40.4	12.4	30.8	5.17	41.8	13.2	30.0	5.32	1.09	0.286	0.725	0.122
	Pontine nuclei	16.5	6.09	14.9	7.33	18.0	7.63	10.2	2.17	0.682	0.115	0.642	0.0998
	Dorsal raphe	18.2	4.91	18.2	6.14	41.0	17.0	19.9	4.29	0.862	0.154	0.743	0.0925
Slice V	Parietal cortex	49.2	13.6	35.5	8.60	41.1	9.79	52.3	5.71	1.08	0.211	0.992	0.168
	Visual cortex	45.8	6.08	32.5	7.00	39.2	13.7	45.5	4.76	0.816	0.112	0.981	0.195
	Brainstem	32.8	4.11	29.4	3.77	23.7	7.19	26.7	6.59	2.45	0.858	2.42	1.14
	Cerebellum	17.1	7.10	16.8	8.87	32.7	11.7	18.1	6.57	0.524	0.133	0.696	0.118
Slice VI	Visual cortex	24.6	7.31	35.7	2.76	46.7	8.62	45.5	5.36	0.829	0.0723	0.679	0.0122
	Cerebellum	55.4	8.47	64.6	13.8	52.0	5.65	40.3	2.57	0.941	0.0759	1.24	0.379

Results are presented as average and SEM

^aEach group received their respective dose material as a constant i.c.v. infusion over 6 weeks (n = 4 per group). All brain samples were taken at the end of the 6 week infusion.

^bEach monkey received four weekly i.v. injections of this dose material (n = 5). All brain samples were taken a week after the fourth dose was administered.

^cIndicates the depth within the brain from which the samples were harvested. The slice number refers to the depth as indicated in Figure 3.

^dIndicates which brain region was in the samples within a given brain slice.