Figure 5.

Effects of rubiarbonone C on MAPK components, such as ERK1/2, p38 and JNK. Serum‐deprived VSMCs were treated with rubiarbonone C (5–20 μM) for 24 h followed by stimulation with 25 ng·mL⁻¹ PDGF BB for 10 min. After stimulation, cells were lysed and the lysates subjected to Western blotting using the antibodies indicated. The bands of phosphorylated proteins were normalized to those of total protein expression. Means ± SEM (n = 5 for each experimental group). The gel images shown are representative of those obtained from five independent experiments. *P < 0.05 versus PDGF BB alone.