Figure 2.

The expression of COX‐2 and mPGES‐1 and release of 6‐keto‐PGF_1α (stable metabolite of PGI₂) and PGE₂ in IMA or SV following 18 h incubation under ‘Normal’ or ‘Inflam’ (inflammatory) conditions. A representative image of Western blot is presented (A). Histograms represent Western blot quantification of COX‐2 (B) and mPGES‐1 (C) corresponding bands. Optical density (OD, arbitrary units) was quantified by Scion Image and normalized by actin. The release of 6‐keto‐PGF_1α (D) and PGE₂ (E) in organ culture supernatant after 18 h incubation were expressed as ng·(mg tissue wet weight) ⁻¹. * P < 0.05 significantly different as indicated; Student's t‐test. Values are means ±SEM derived from (n) different patients.