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. 2017 Oct 23;11:79. doi: 10.3389/fncir.2017.00079

Figure 2.

Figure 2

Disrupting highwire (hiw) increases waking. (A–C) Reducing hiw in c929 expressing cells using hiwRNAi28163, hiwRNA36085, or UAS-hiwDN disrupts sleep. A Genotype (3) X Time (24) repeated measures analysis of variance (ANOVA) revealed a significant Genotype × Time interaction, ANOVA F(46,11058) = 4.96; p = 9.9E-16, F(46,1196) = 5.97; p = 9.9E-16, and F(46,996) = 5.26; p = 9.9E-16, respectively. (D) Disrupting hiw reduces average sleep bout duration at night, one-way ANOVA for Genotype F(6,93) = 6.58, p = 7.97E-006; *p < 0.05 Modified Bonferroni test. (E) c929-GAL4>UAS-hiwDN flies exhibit normal values for the Time to complete Test (TCT), the Photosensitivity Index (PSI) and the Quinine Sensitivity Index (QSI) that are not statistically different from both c929-GAL4/+ and UAS-hiwDN/+ parental controls, one-way ANOVA F(2,26) = 0.22, p = 0.8, ANOVA F(2,14) = 6.3, p = 0.01, ANOVA F(2,14) = 0.73, p = 0.9, respectively; *p < 0.05 modified Bonferroni test). (F) Baseline learning is reduced in flies expressing UAS-hiwDN in c929 expressing cells one-way ANOVA F(2,26) = 3.54, p = 0.042; *p < 0.05 modified Bonferroni test).