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. 2017 Oct 23;11:79. doi: 10.3389/fncir.2017.00079

Figure 8.

Figure 8

Knocking down Rdl in the lLNvs protects flies from sleep-loss induced increases in synaptic markers. (A) Representative images of PDF immunohistochemistry of c929-GAL4/+>UAS-hiwRNAi/+ flies reveals an increase in the number (upper panels) and size (lower panels) of varicosities compared to c929-GAL4/+ and UAS-hiwRNAi/+ parental controls. (B) Quantification of terminal number expressed as a percentage of GAL4/+ (upper panel) revealed a significant One-way ANOVA for genotype F(2,30) = 6.0, p = 0.006, n = 9–12 each group; *p < 0.05 modified Bonferroni correction. Quantification of bouton size (lower panel) revealed a significant one-way ANOVA for genotype F(2,30) = 17.6, p = 1.07E-05, n = 9–12 each group; *p < 0.05 modified Bonferroni correction. (C) Representative images of PDF immunohistochemistry of c929-GAL4/+>UAS-RdlRNAi/+ flies does not show any change in the number (upper) or size (lower) of PDF positive punctae compared to c929-GAL4/+ and UAS-RdlRNAi/+ parental controls. (D) Quantification of terminal number and size did not reveal a significant one-way ANOVA for number or size F(2,17) = 0.2, p = 0.79 and F(2,17) = 1.9, p = 0.18, respectively; n = 7 each group.