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. 2017 Oct 23;8:2061. doi: 10.3389/fmicb.2017.02061

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Copyright © 2017 Machida, Bakku and Suzuki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Modification of the Shine–Dalgarno (SD) sequence upstream of the coding sequence of the methyltransferase WP_048472121. (A) The native genomic sequences encoding the FAD-binding oxidoreductase (FADO) WP_08472120 and the SAM-dependent methyltransferase WP_048472121 was shown. (B) The genomic sequences substituted the two nucleotides in upstream of WP_048472121 without alteration of the encoded polypeptide sequence was shown. The substituted nucleotides are shown by a bold, red-colored font. (C) The genomic sequences inserted 14 nucleotides including the canonical SD sequence from Escherichia coli between FADO and WP_048472121. The inserted nucleotides are shown by a bold, blue-colored font. The translated amino acid sequences are shown. ^∗∗∗Indicates the stop codons. –15 and –5 indicate positions from the translational start codon of WP_048472121. Putative SD sequences were underlined.