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. 2017 Oct 23;8:1346. doi: 10.3389/fimmu.2017.01346

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Copyright © 2017 Ichii, Kimura, Okamura, Horino, Nakamura, Sasaki, Elewa and Kon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of IL-36α in vitro. (A) Il1f6 mRNA expression in 209/MDCT cells after stimulation with various agents for 24 h. A significant difference with PBS control (Cont) is indicated by *(P < 0.05) or **(P < 0.01) (n = 4). (B) Immunofluorescence in 209/MDCT cells after PBS or LPS exposure for 24 h. Green, red, and blue indicate pan-actin, IL-36α, and nuclei, respectively. (C) Immunoblotting for IL-36α and β-actin using cell lysates from 209/MDCT cells after PBS or LPS exposure for 24 h.