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. 2017 Oct 23;8:1346. doi: 10.3389/fimmu.2017.01346

Figure 7.

Figure 7

IL-1RL2 expression in the mouse kidney and induction of IL-6 production by IL-36α stimulation in vitro. (A) Immunofluorescence for IL-36α and IL-1RL2 in Cont and unilateral ureter obstruction (UUO) kidneys at 48 h. Green, red, and blue indicate IL-1RL2, IL-36α, and nuclei, respectively. Arrows indicate the dotted or linear IL-1RL2+ reactions. G, glomerulus; Lu, lumen. (B) Immunofluorescence for IL-36α and IL-1RL2 in Cont and UUO kidneys at 48 h. Green, red, and blue indicated IL-1RL2, IL-36α, and nuclei, respectively. Arrows and arrowheads indicate the IL-1RL2+ interstitial cells and platelets, respectively. G, glomerulus. (C) Relative mRNA expression of Il1rl2 in Cont and UUO kidneys. Values = mean ± SE. n ≥ 4 (kidneys). (D) Relative mRNA expression of Il1rl2 in mouse cell lines. Values = mean ± SE. n = 4. A significant difference with other cell lines is indicated by **(P < 0.01). Mes, mesangial; Epi, epithelial; CD, collecting duct; Fibro, fibroblast. (E) Relative mRNA expression of Il1rl2 and Il6 and medium IL-6 levels in IL-36α-exposed NIH3T3 cells. Values = mean ± SE. n = 4. A significant difference with 0 h is indicated by *(P < 0.01) or **(P < 0.01).