Table 3.
Gene ontology (GO) analysis of genes showing altered expression in the kidneys of wild-type (WT) and IL-36α knockout (KO) mice after unilateral ureter obstruction (UUO) at 48 h.
| Comparison | Ontology aspects | GO term | Cluster frequency (%) | Genome frequency (%) | Corrected P-value |
|---|---|---|---|---|---|
| Downregulated genes in UUO-treated kidney of IL-36α-KO mice | Process | Inflammatory response | 8.50 | 2.50 | 0.00088 |
| Acute inflammatory response | 3.50 | 0.50 | 0.0056 | ||
| G-protein coupled receptor signaling pathway | 17.40 | 7.80 | 0.00044 | ||
| Sensory perception | 15.80 | 7.60 | 0.00931 | ||
| Function | Signaling receptor activity | 17.40 | 8.50 | 0.00086 | |
| Signal transducer activity | 18.90 | 9.70 | 0.00103 | ||
| Receptor activity | 18.10 | 9.40 | 0.00217 | ||
| Molecular transducer activity | 18.10 | 9.40 | 0.00217 | ||
| Transmembrane signaling receptor activity | 16.20 | 8.20 | 0.00334 | ||
| Transmembrane receptor activity | 16.20 | 8.30 | 0.00548 | ||
| Component | NA | ||||
| Two hundred and fifty nine genes were downregulated in the kidneys of IL-36α-KO mice compared with that that in the kidneys of WT mice; 24,424 genes were examined; Bonferroni correction for P-values. NA: not annotated | |||||
| Upregulated genes in UUO-treated kidney of IL-36α-KO mice | Process | Sensory perception of chemical stimulus | 29.00 | 5.90 | 1.50E−34 |
| Sensory perception of smell | 23.80 | 4.60 | 1.28E−28 | ||
| Sensory perception | 30.00 | 7.60 | 1.96E−28 | ||
| Neurological system process | 31.60 | 9.20 | 1.01E−25 | ||
| G-protein coupled receptor signaling pathway | 27.40 | 7.80 | 4.29E−22 | ||
| System process | 33.20 | 11.70 | 9.47E−21 | ||
| Detection of chemical stimulus involved in sensory perception | 5.20 | 1.20 | 0.00132 | ||
| Detection of chemical stimulus | 5.20 | 1.30 | 0.0041 | ||
| Signal transduction | 34.90 | 23.50 | 0.00418 | ||
| Multicellular organismal process | 44.60 | 32.70 | 0.0079 | ||
| Function | Olfactory receptor activity | 23.80 | 4.50 | 5.13E−30 | |
| Receptor activity | 31.30 | 9.40 | 7.54E−25 | ||
| Molecular transducer activity | 31.30 | 9.40 | 7.54E−25 | ||
| Transmembrane signaling receptor activity | 29.00 | 8.20 | 8.46E−25 | ||
| Transmembrane receptor activity | 29.00 | 8.30 | 3.76E−24 | ||
| Signaling receptor activity | 29.00 | 8.50 | 2.26E−23 | ||
| Signal transducer activity | 29.30 | 9.70 | 4.62E−20 | ||
| Odorant binding | 10.70 | 1.90 | 1.16E−13 | ||
| Pheromone binding | 2.60 | 0.40 | 0.00356 | ||
| Serine-type endopeptidase inhibitor activity | 2.90 | 0.50 | 0.00365 | ||
| Endopeptidase inhibitor activity | 3.60 | 0.80 | 0.00506 | ||
| Endopeptidase regulator activity | 3.60 | 0.80 | 0.00708 | ||
| Peptidase inhibitor activity | 3.60 | 0.80 | 0.00814 | ||
| G-protein coupled receptor activity | 6.80 | 2.60 | 0.00961 | ||
| Component | Integral component of membrane | 37.50 | 23.40 | 2.69E−06 | |
| Intrinsic component of membrane | 37.80 | 24.00 | 6.19E−06 | ||
| Membrane part | 39.10 | 28.60 | 0.00736 | ||
| Three hundred seven genes were upregulated in the kidneys of IL-36α-KO mice compared to that in the kidneys of WT mice; 24,424 genes were examined; Bonferroni correction for P-values | |||||