Fig. 2.

S1P₁ activation after treatment with an S1P₁ antagonist in live mice. a S1P₁ luciferase signaling (S1P₁LS) mice were serially injected with vehicle, followed by the S1P₁ antagonist W146 (5 or 10 mg/kg, ip injection). b Representative repeat bioluminescence images of the same mice (top, supine view of mouse # 1, 2, 3; bottom, prone view of mouse # 4, 5, 6) comparing the effects of vehicle with those of W146 (10 mg/kg), 0.5 h after injection. BLI was also performed 1 day after the W146 injection (post W146). Red open rectangles representing ROI were positioned around cephalic, thoracic, and epigastric regions. c, d The bioluminescence activity was quantified by determining the total flux (photons/sec; p/sec) in each ROI. Data acquired from supine (c) and prone (d) views are shown. Data represent the mean ± SEM. n = 6 for each group. P values were determined by one-way ANOVA followed by Bonferroni’s multiple comparisons test; **P ≤ 0.01, ***P ≤ 0.001