Fig. 1.
Expression of miR-34a in hyperoxia exposed NB lungs and type 2 cells. a Representative graphs showing miR-34 expression in WT NB mice exposed to hyperoxia for 2, 4, and 7 days after birth and in the BPD model. b Primary miR-34a expression is shown in hyperoxia exposed and BPD murine lung tissue as compared to controls. c Freshly isolated type 2 epithelial cells were used for measuring miR-34a expression in room air (RA) and after 4 h and 16 h HYP (95% O2). d, e MLE12 cells were exposed to different concentrations of oxygen (21, 40, 60, and 95%) for 24 h and 48 h, respectively. NB: newborn; RA: room air; HYP: hyperoxia. A minimum of four animals were used in each group. *P <0.05, **P <0.01, ***P <0.001, ****P <0.0001, compared with controls; 1-way ANOVA