Fig. 3.

miR-34a specifically controls Ang1-Tie2 expression in lung epithelial cells. a MLE12 cells were transfected with different concentrations of miR-34a mimic in RA and western blots for Ang1 and Tie2 expression were performed. b The wild-type Ang1 3′ UTR reporter vector was co-transfected into the MLE12 cells with either the N.C. mimic or miR-34a mimic c The WT Tie2 3′ UTR reporter vector was co-transfected into the MLE12 cells with either the N.C. mimic or miR-34-a mimic. d The wild-type Ang1 3′ UTR reporter vector was co-transfected into the MLE12 cells with either the scrambled or miR-34a inhibitor. Ang1: Angiopoietin 1; WT: Wild-type; RA: room air; N.C.: Negative control. Values are means + SEM of a minimum of four observations. *P <0.05, **P <0.01, 2-way ANOVA, Tukey’s