Fig. 2.

Specific sensing of C/N- or S-precursors for Cys results in distinct transcriptome reprogramming and specific translation regulation. a Venn diagrams and b–e functional category analysis (p < 0.05, FDR < 0.25) for transcriptional response in roots of 7-week-old serat tko and sir1-1 plants. The compared sets of genes contained transcripts, which were significantly (p < 0.05) up- or downregulated by more than 1.25-fold in sir1-1 or serat tko in comparison to the wild type. The details of the highlighted categories with underlines are further presented in Supplementary Figs. 2 and 4. f Specific downregulation of 18s rRNA in response to sulfide limitation (n = 3, mean ± s.e.m., one-way ANOVA, *p < 0.05). g TOR and GCN2 activity was determined by antibodies against S6K-p, S6K (apparent sizes: 52 kDa) and eIF2α-p (apparent size: 43 kDa) and h calculated by the ratio of S6K-p/S6K and eIF2α-p/LC in x-fold of wild type (n = 3, mean ± s.e.m., one-way ANOVA, *p < 0.05)