Figure 3.

Determination of the ethidium bromide efflux by a semi-automated fluorometric method. Klebsiella pneumoniae isolates were saturated with 50 μM ethidium bromide and efflux measured by fluorometry at 37°C for 50 min with an excitation and emission wavelengths of 518- and 606-nm, in the presence or absence of glucose and CCCP. Black arrows indicate when glucose (50 mM) was added after 3 min of initial reading.