Fig. 3.

Low numbers of thioflavin-S-positive amyloid plaques were detected in both the cortex and hippocampus of 4- to 5-month-old APP/PS1 transgenic mice but not in their wild-type littermates. Representative fluorescent thioflavin-S stained micrographs of 20-μm-thick frozen brain sections showing single thioflavin-S stained plaques in the cortical (a) and hippocampal (b) brain regions from 4- to 5-month-old APP/PS1 mice; no thioflavin-S-positive plaques were observed in any of the brain sections examined in wild-type mice (data not shown). c The numbers of thioflavin-S plaques/field were significantly lower in the CA1 region than in the cortex (*p = 0.0152; n = 6 per group); all other statistical comparisons were non-significant (p > 0.05). Statistical analyses were conducted using either a Mann–Whitney test or unpaired t test and presented as mean ± SEM. Scale bar 200 μm