Fig. 4.

Functional effects of BRD7 overexpression and knockdown in endothelial cells. a, b Full-length BRD7 (BRD7-FL) and bromodomain deletion mutant (BRD7-dBr) cloned in pcDNA3 [27] were ectopically expressed in RF24. Control cells were transfected with empty pcDNA3. This resulted in seven to tenfold increased in BRD7 expression (a). BRD7 overexpression results in inhibition of EC proliferation as measured by ³H-thymidine incorporation assay (b). c Overexpression of BRD7 and BRD7-dBr results in a reduced fraction of adherent cells. Cells were transfected with GFP-tagged BRD7 expression constructs or empty pEGFPN1 vector, which allowed easy identification of successfully transfected cells. *p < 0.05, **p < 0.01 ANOVA. d–f Knockdown of BRD7 by siRNA in HUVEC (d) does not affect proliferation of cells (e). Migration toward conditioned medium of siBRD7-treated cells was enhanced (f). **p < 0.01 t test. All data are presented as mean ± SEM