Fig. 5.

BRD7 expression modulation affects angiogenic and inflammatory gene expression. a–d Full-length BRD7 (BRD7-FL) and bromodomain deletion mutant (BRD7-dBr) were ectopically expressed in RF24, which resulted in a bromodomain-dependent increase in TNF-α expression (a), NFkB activation (b), and ICAM1 mRNA and protein (d). Expression of CXCL1, CXCL6 and NOTCH1 is only slightly affected (c). e–g mRNA expression changes are reversed upon knockdown of BRD7, although ICAM1 protein expression was not affected. *p < 0.05, **p < 0.01 ANOVA. All data are presented as mean ± SEM