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. 2017 Oct 27;7:14223. doi: 10.1038/s41598-017-14671-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative expression of MdSPL7 and MdSPL26 in wild and transgenic lines of Nicotiana benthamiana. The expression constructs MdMIR156 (a5 or a12) and MdSPL (MdSPL7 or MdSPL26) were mixed and transformed simultaneously into N. benthamiana by injection. The experiment was performed with three replicates injected with five plaques. The pBI121 vector harboring the GUS reporter gene was also injected to confirm the conversion efficiency. GUS was used as the reference gene. Error bars represent the SD of three experimental replicates.