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. 2017 Oct 27;7:14246. doi: 10.1038/s41598-017-14475-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic and genetic analysis of eGFP transduction. (a) Freshly hatched G₁ offspring, one GFP-positive chicken right, wt chicken left. (b) PCR detection of eGFP transduction in the semen of roosters 649, 659, and 675 and in the GFP-positive G₁ chicken No. 146. The eGFP-specific PCR fragment is 525 bp in length.