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. 2017 Oct 27;7:14259. doi: 10.1038/s41598-017-14641-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The NMR spectrum of NCYC 65 in sucrose (a), sucrose standard (b), glucose standard (c) and fructose standard (d). Spectrum (a) contains the same peaks as spectrum (c) and spectrum (d) which means that glucose and fructose are present in spectrum (a). No similarity was identified between spectrum (a) and spectrum (b) which means no sucrose in spectrum (a). The graph on the top right shows the quantification of glucose, fructose and sucrose of sample “NCYC 65 in sucrose” and sample “sucrose control”. This illustrates sucrose was completely degraded into glucose and fructose by yeast strain NCYC 65. Results were collected from triplicates.