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. 2017 Oct 27;7:14235. doi: 10.1038/s41598-017-14695-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of wrky43-1 mutant (ET5604) and WRKY43 expression analysis. (A) wrky43-1 with transposon insertion in the second exon. Exons (black) and intron (black line) are indicated. Two pairs of primers (43_f and 43_rev; DS and 43_rev) were used to check transposon-insertion and are indicated with arrows. (B) PCR screen for transposon insertion in Ler-0 wild type and wrky43-1 mutant. (C) RT-PCR of Ler-0 wild type and wrky43-1 mutant displayed no full length WRKY43 transcript in wrky43-1 silique cDNA. Actin 2 was used as the control. (D) RT-qPCR of WRKY43 transcript levels in Ler-0 wild type, wrky43-1 mutant, complementation lines wrky43-1:WRKY43 and wrky43-1:WRKY43 -strepII and overexpression WRKY43 line p35S-YFP-WRKY43 (mean ± SE; n = 3). (E) Expression profiles of WRKY43 in various A. thaliana tissues. WRKY43 expression was detectable in silique stages 6–10 and in roots (mean ± SE; n = 3). (F) Confocal images of YFP-WRKY43 in transiently transformed N. benthamiana leaves (scale bar = 10 µm).