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. 2017 Oct 27;7:14261. doi: 10.1038/s41598-017-14496-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of the assembly products. M1: TAKARA 1 kb DNA ladder; M2: TAKARA λ-HindIII digest. (a) Plasmids of pLDR-BF1(1)-(24) screened by PCR amplification directly from 24 colonies with primers B1-F and B1-R; the predicted band was 2025 bp. (b) Plasmids of pLDR-BF1~BF2(1)-(24) screened by PCR amplification directly from 24 colonies with the primers B2-F and B2-R; the predicted band was 1687 bp. (c) Plasmids of pLDR-BF1~BF3(1)-(24) screened by PCR amplification directly from 24 colonies with the primers B3-F and B3-R; the predicted band was 1362 bp. (d) 1: The final vector of pLDR-BF1~BF3 digested by BamHI; the predicted band was 14777 bp. 2: The final vector of pLDR-BF1~BF3 digested by SalI; the predicted bands were 12629 bp and 2148 bp.