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. 2017 Oct 27;7:14272. doi: 10.1038/s41598-017-14612-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bleomycin-induced pulmonary fibrosis was associated with ER stress activation. Mice with intratracheal administration of bleomycin (2 U/kg) or saline (vehicle) were sacrificed 14 days later and the lung specimens were harvested for (A Left) histological analysis with HE, Picro Sirius red and Masson’s trichrome staining followed by (A Right) quantification, (B) The results of total collagen assay, (C) Western blot analysis (Cropped blots are displayed; Full-length blots are presented in Supplementary Figure, labeled Figure S1), and (D) immunofluorescence for the expression of proteins associated with ER stress activation.