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. 2017 Oct 27;7:14252. doi: 10.1038/s41598-017-13882-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Perfusion-cultured human cells were directly involved in the formation of blood vessels. Patches were analysed after 28 days in vivo. (A) Representative immunofluorescence images of EC (Ve-Cad; red), pericytes (NG2; grey) and HuNu (green). White arrows indicate human cells directly involved in the formation of blood vessel Ve-Cad⁺ or NG2⁺. Quantification of the human cells Ve-Cad⁺ (B) and NG2⁺ (C) normalized over the analyzed area (n donor = 3). (D) 3D reconstruction of immunofluorescence images of EC (Ve-Cad; red), basal lamina (Laminin; grey) and HuNu (green). Nuclei were stained with DAPI (blue in A,D). *p < 0.05. Scale bar = 20 μm.