Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 27;7:14252. doi: 10.1038/s41598-017-13882-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Pericyte depletion decreased cell proliferation and in vitro pre- and in vivo early vascularization. Patches were analysed after 3 days in vivo. (A) Representative in vitro immunofluorescence images of proliferating cells (Ki-67; green) and pericytes (CD146; red). White arrows indicate proliferating CD146⁺ cells. Scale bar = 20 μm. (B) Representative in vitro immunofluorescence images for EC (CD31; green) and basal lamina (laminin; red). Scale bar = 20 μm. (C) Representative in vivo immunofluorescence for human cells (HuNu; green) and EC (Ve-Cad; red). Nuclei were stained with DAPI (blue in A–C). Dashed line outlines the border between the patch and the rat tissue (identified by the *). White arrows indicate human cells. Scale bar = 50 μm. (D) Quantification for the HuNu⁺ cells normalized over the analysed area. (E) VLD quantification normalized over the analysed area. *P < 0.05 (n donor = 3).