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. 2017 Oct 27;7:14233. doi: 10.1038/s41598-017-14601-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative real-time PCR analysis of genes involved in heavy metal transport (HvHMT1, HvHMT2, HvHMT3 and HvHMT4), ABA biosynthesis (HvZEP1, HvZEP2, HvNCED1, HvNCED2, HvAO1, HvAO2 and HvAO3) and ABA catabolism (HvABA8′OH-1, HvABA8′OH-2, HvABA8′OH-3, HvBG1 and HvBG2) in non-irradiated (0- Gy) and irradiated (50- Gy) highland barley seedlings with 500 µM Pb(NO₃)₂ (a) and 75 µM CdCl₂ (b) treatment. The results are reported as the relative expression of each gene transcripts with respect to internal standard. The relative value for the expression of each gene in non-irradiated sample was set as 1. Vertical bars represent the mean ± SD of three independent replications (n = 3). Significant difference between non-irradiated and irradiated sample for each gene is indicated with * at p < 0.05, ** at p < 0.01 and *** at p < 0.001, according to paired-sample t-test.