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. 2017 Oct 27;7:14233. doi: 10.1038/s41598-017-14601-8

Table 1.

The accession number and primer sequence of each gene in the real-time quantitative RT-PCR assay.

Gene Oligo Sequence
Forward Reverse
HvHMT1 5′-CAGAAGAAGGTGCTCAAGAC-3′ 5′-GTTATACGAGGAAGTGGACC-3′
HvHMT2 5′-CAAGATCAAGCAGGAGGAAT-3′ 5′-GATGAGAGACCGCTTGATC-3′
HvHMT3 5′-GTGGAGATGCAGATGAACAT-3′ 5′-CAACACCTTCTTCTGGCTC-3′
HvHMT4 5′-CTCAAGGACAACAAGATGAC-3′ 5′-GACCTTCTTGTCGTCTTTCT-3′
HvZEP1 5′-GCGAGAGGCGGGGGAGAAGT-3′ 5′-TGGTGACAAGGGGTGGCTGAAG-3′
HvZEP2 5′-CTTCCTGGCTCGTCGGTTCGTC-3′ 5′-GCTGGGAGTGGAGGGCGTGTAA-3′
HvNCED1 5′-CCAGCACTAATCGATTCC-3′ 5′-GAGAGTGGTGATGAGTAA-3′
HvNCED2 5′-CATGGAAAGAGGAAGTTG-3′ 5′-GAAGCAAGTGTGAGCTAAC-3′
HvAO1 5′-TCCCTGCGCCAATGACACA-3′ 5′-GTTGACGGCACCGGAATCTTG-3′
HvAO2 5′-TCTTGTGCGCCCCTCATCTTCA-3′ 5′-TCCCTTGCCCAACCTCAACACCT-3′
HvAO3 5′-GTGCGAGCGCCAGGTGATGT-3′ 5′-TCGGCGCTGTGGAGGTTCTTT-3′
HvABA8OH-1 5′-AGCACGGACCGTCAAAGTC-3′ 5′-TGAGAATGCCTACGTAGTG-3′
HvABA8OH-2 5′-GAGATGCTGGTGCTCATC-3′ 5′-ACGTCGTCGCTCGATCCAAC-3′
HvABA8OH-3 5′-CCGGCGGCAGCGTCTTCT-3′ 5′-GTGTTGCCGTCCTGGGTGTCC-3′
HvBG1 5′-CCTACGTCGCCGCCCATAACA-3′ 5′-GCCCGTCTTGCAGCCAGGATA-3′
HvBG2 5′-GCCGGTGGGAACTCAGCAACAG-3′ 5′-GTCGGCAGGTGAGTCGGTAGCA-3′
AY145451 5′-GACTCTGGTGATGGTGTCAGC-3′ 5′-GGCTGGAAGAGGACCT CA-3′