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. 2017 Oct 27;7:14247. doi: 10.1038/s41598-017-14178-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EPS116 induced apoptosis in CT26 cells via death receptor Fas and its ligand Fasl. (A,B) Relative expression levels of target genes; (C) Protein expression levels of Fas and Fasl; (D) Density of target genes normalized to that of β-actin. For clarity lanes were cropped from a single immunoblot. Each sample were treated with different EPS116 concentrations (0–800 μg/mL) for 48 h before cell lysis.