Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 27;7:14274. doi: 10.1038/s41598-017-14423-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Generation of transgenic Pax6-Norrin mice. (A) Schematic drawing of the transgene. (B) Northern blot analysis of Norrin mRNA in RNA of retinae from three transgenic lines (Pax6-Norrin-04, -06 and -69) and wild-type littermates (WT) at P2. Integrity of loaded RNA was controlled by methylene blue staining. (C) Western blot analysis for β-catenin in retinae from transgenic line Pax6-Norrin-04 and wild-type littermates (WT) at P22. β-catenin levels were analyzed by densitometry, normalized to GAPDH and plotted as x-fold to levels of wild-type controls (mean ± SEM; n = 9; **p < 0.01). (D,E) Immunostaining for β-catenin (green) in P22 retinae of wild-type and transgenic Pax6-Norrin-04 mice in the FVB/N/CD-1 background (D) and crossed with DBA/2J mice (E). Nuclei are labeled with DAPI (blue). GCL. ganglion cell layer; INL. inner nuclear layer; ONL. outer nuclear layer; Scale bars: 20 μm.