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. 2017 Oct 23;8:744. doi: 10.3389/fphar.2017.00744

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Copyright © 2017 Martínez-Pinilla, Varani, Reyes-Resina, Angelats, Vincenzi, Ferreiro-Vera, Oyarzabal, Canela, Lanciego, Nadal, Navarro, Borea and Franco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dose-response effect of CBD on ERK1/2 phosphorylation. HEK-293T cells transfected with human CB₂R were stimulated with 100 nM of JWH133 agonist in the presence of CBD. ERK1/2 phosphorylation levels were determined by using AlphaScreen^®SureFire^® kit. Data are normalized and expressed as a percentage of over-basal response (mean ± SEM). Significant differences were analyzed on data from three different experiments; one-way ANOVA and Bonferroni’s multiple comparison post hoc test were used for statistical analysis (F_4,10 = 11, p < 0.01) (^∗p < 0.05, ^∗∗p < 0.01, versus 0 nM CBD treatment).