Figure 1.

A) Multiplex PCR for detecting ETEC pathotype. Lane M: molecular weight marker. Lanes 1 and 11: Negative control. Lane 2 and 7: elt (322 bp). Lanes 3 and 8: est (147 bp). Lanes 4 and 9: elt (322 bp) and est (147 bp). Lane 5, 6 and 10: elt and est-gene negative E. coli isolates. (B)Multiplex PCR for detecting EAEC, EIEC, and EPEC pathotypes. Lanes 2,3,4,5,6,8 and 9: EAEC-aggR (254 bp) and aspU (282 bp). Lanes: 1 and 7: aggR, ipaH, and aspU-gene negative samples.Lane 10: Negative control. (C) Multiplex PCR for detecting EAEC, EIEC, and EPEC pathotypes. Lane M: Molecular weight marker. Lanes 1, 2, 3, 4:EIEC-ipaH-gene (600 bp). Lane 5,6 and 7: ipaH-gene negative sample. Lane 8: Negative control. (D) Singleplex PCR for detecting shigellaipaH-gene. Lane M: Molecular weight marker. Lanes 1,2,3,4,5,6,7,8, 9, 10 and 11: ipaH(619 bp). Lane 12: Negative control