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. 2017 Oct 27;7:14199. doi: 10.1038/s41598-017-14230-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of α1D protein expression in colon tissues microarrays. (A) Proportion of samples showing no (white), low (light gray), medium (black grey) or strong (black) α1D staining in normal mucosa, compared with adenoma and adenocarcinoma samples. The number of samples per condition is indicated in brackets. α1D staining was stronger in adenoma (χ ², p < 0.04) and adenocarcinoma tissues (χ ², p < 0.001) compared to normal tissues and in adenoma tissues compare to adenocarcinoma tissues (χ ², p < 0.03). (B) α1D protein of CaV1.3 expression in normal mucosa (left), adenoma (center) and adenocarcinoma (right) from a same sample (1:100 dilution, Red revelation, Hematoxylin counter-coloration). Magnification, ×100 (top), ×300 (bottom). (C) Proportion of samples showing no (white), low (light gray), medium (black grey) or strong (black) α1D staining according to the state of differentiation (right) or stage (left) of adenocarcinoma samples. No difference was observed according the state of differentiation of adenocarcinoma samples (Kruskall Wallis test, p = 0.5). An influence of pT stages was observed on α1D staining (Kruskall Wallis test, p = 0.008).