Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 27;7:14199. doi: 10.1038/s41598-017-14230-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Lack of detectable voltage-dependent Ca²⁺ currents and cellular localization of α1D protein. (A,B) Current records during 600 ms pulses in the range of −70 to 110 mV from holding potential of −100 mV. Representative examples of current recording in presence of barium (20 mM) and Bayk 8644 (50 µM) in broken (A) and perforated (B) patch configurations. Whole cell currents are obtained in normal condition (B.1) and during the migration process (B.2). Arrows indicates zero current. (C) Fluorescence-activated cell sorter (FACS) analysis of α1D protein in HCT116 cell line permeabilized or not. Control staining is shown in light grey, and dark grey represent staining with anti-CaV1.3. Graph represents the percentage of cells (N = 6) expressing α1D protein in permeabilized (Total) and not permeabilized cells (Plasma membrane cell surface). (D) Subcellular distribution of α1D protein in HCT116 cell line. A primary anti-CaV1.3 antibody was used with a secondary AlexaFluor 488-conjugated anti-rabbit (green color). The short arrows indicate the location α1D protein in the edges membranes, and the long arrows show the presence of α1D protein in the cytosol.