Figure 1.

Astrocyte Ca²⁺ imaging and observed response variability. (A) (Left) A 12 μm z-stack (centered around the imaging plane) of tdTomato indicating the location of three recorded astrocytes relative to the pipette (shown with arrow), which is also visible since it contains Alexa Fluor 594. The cell numbers correspond to those in (B). (Right) Time series of intracellular Ca²⁺ responses to focal application of ATP (application duration, 63 ms) from a glass pipette (white dotted line) in a mouse acute brain slice. Examples of selected ROIs are shown for the soma, large processes, and small processes (cyan lines in the 20 s frame). These ROIs correspond to the traces of cell 1, trial 1 in (B). Scale bars, 20 μm. (B) Example traces of simultaneous responses from different subcompartments of three cells (same cells shown in A), in two different ATP application trials.