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. Author manuscript; available in PMC: 2017 Dec 6.
Published in final edited form as: Anal Chem. 2016 Nov 22;88(23):11654–11662. doi: 10.1021/acs.analchem.6b03227

Figure 4.

Figure 4

Although E-DNA signal gain is a strong function of the density with which the DNA probes are packed on the surface,11,24 the amplitude/frequency pairing at which this is observed is itself independent of probe density. To see this, we recorded signal gain maps for the aminoglycoside sensor (with a methylene blue reporter) fabricated by depositing the probe at concentrations of either (A) 20 nM or (B) 500 nM. These resulted in 1.1-fold and 1.4-fold decreases in signal gain, respectively, in comparison to sensors fabricated using a more optimal probe concentration of 200 nM (Figure 2A). The optimal amplitude/frequency pairing, however, is effectively indistinguishable across all three packing densities.