Skip to main content
. 2017 Oct 27;37(22):e00265-17. doi: 10.1128/MCB.00265-17

FIG 1.

FIG 1

RNF20 is downregulated in ccRCC. (A) qRT-PCR analysis of RNF20 mRNA expression in patient-matched normal kidney (n = 9) and ccRCC tumor (n = 9) samples. RNF20 mRNA levels were normalized to those in matched normal kidney samples. (B) Normalized RNA-seq reads of RNF20 in normal kidney (n = 72) and ccRCC tumor (n = 533) samples. RNA-seq data were obtained from TCGA. (C) RNF20 expression in ccRCC tumors was analyzed according to tumor stages. Significance versus normal kidney samples: ##, P < 0.01; ###, P < 0.001. (D) Representative ccRCC tissue microarray used for IHC with RNF20 antibody. (E) IHC staining of patient-matched adjacent normal kidney and ccRCC tumor tissues. Representative tissue sections stained for RNF20 are shown. Bar, 100 μm. (F) RNF20 protein expression levels in normal kidney cell lines, such as HRCE and HEK293, and ccRCC cell lines, including ACHN, A498, and Caki-2, were determined by Western blotting. (G) RNF20 mRNA expression in normal kidney and ccRCC cell lines was determined by qRT-PCR. Significance versus HRCE: ##, P < 0.01.