FIG 2.

RNF20 suppresses cell growth in ccRCC but not in normal kidney cell lines. (A) ACHN and A498 ccRCC cells were infected with adenovirus expressing GFP alone (−) or Myc-RNF20(+). After infection for 24 h, total cell lysates were subjected to Western blotting. (B) ACHN and A498 ccRCC cells were infected with adenovirus expressing GFP alone (Mock) or RNF20, and proliferation was monitored using the Cell Counting Kit-8 (CCK-8) assay. (C) ACHN and A498 ccRCC cells were transfected with siControl or siRNF20, and RNF20 expression was determined by Western blotting. (D) ACHN and A498 ccRCC cells were transfected with siControl or siRNF20, and relative growth rates were determined using the CCK-8 assay. (E) HRCE and HEK293 normal kidney cells were infected with adenovirus expressing GFP alone (−) or Myc-RNF20(+), and cell lysates were examined using Western blotting. (F) HRCE and HEK293 cells were infected with adenoviral RNF20, and cell proliferation was monitored using the CCK-8 assay. (G) HRCE and HEK293 cells were transfected with siControl or siRNF20, and cell lysates were determined by Western blotting. (H) HRCE and HEK293 cells were transfected with siRNF20, and cell proliferation rates were monitored using the CCK-8 assay. Cell proliferation data are presented as the means ± SD from five individual samples. *, P < 0.05; **, P < 0.01; n.s., not significant.