FIG 2.

Inflammation-related genes are downregulated in the collecting duct cells of G2CKO mice. (A) Biological pathway annotation based on IPA. The gene signatures that were affected in G2CKO-CD cells were classified into the indicated pathways. (B) Forty-one inflammation-related genes encoding several chemokines and cytokines were reduced more than 2-fold in G2CKO-CD cells compared with wild-type (WT) control CD cells. The mRNA expression levels in the WT samples were set to 1 in the heat map. (C) Ccl12, Cxcl10, S100a14, and Tnf mRNA are more strongly expressed in the DBA-sorted CD cells than in whole kidney cells 1.5 h after the induction of IRI. The data are derived from the CD cells and whole kidney (Kid) cells of three WT mice. The statistical significance of differences between the whole-kidney cell samples and CD cell samples is indicated (**, P < 0.01; *, P < 0.05). (D) Transcript levels of the 4 cytokine genes were significantly lower in G2CKO-CD cells than in control CD cells under the sham or IRI treatment. The statistical significance of differences between the G2CKO and the control CD cells is indicated (3 mice/group; ***, P < 0.005; *, P < 0.05).